HPLC Optimization Tool - The '2-Parameter' Case

HPLC Optimization Tool

The '2-Parameter' Case for Isocratic, Reversed-Phase Separations (ver. 2.0 beta)

The fundamental goal of this tool is to calculate, for a user-specified particle size and column dead time, the mobile phase velocity and column length that will yield the largest plate count in the time specified (at the pressure limit specified).

Typical ranges for input parameters are shown in parentheses; typical values are entered as inputs by default where appropriate, but are fully adjustable
See superscripts and corresponding footnotes below for more detailed information
We like feedback; please email us at fast2dlc(at)gmail(dot)com with comments, questions, or suggestions
Credits - Plotting on this page uses the 'divgraph.js' package written by Dr. Bob Hansen at St. Olaf College

Inputs

Primary
#1 - (Red)

#2 - (Blue)
Secondary
#1

#2

Column Temperature (^oC) Column Permeability^a (Φ)

Maximum Pressure (bar) Interstitial Porosity (ε_e, 0-1)

Particle Size (μm) Intraparticle Porosity (ε_i, 0-1)

Column Dead Time (t_o, s)

Column Diameter (mm) van Deemter Coefficients (in reduced terms)

A (1-2)

Organic Modfier Fraction (MeCN, 0-1) B (3-20)

Analyte Diffusion Coef. @ 40 ^oC (cm²/s) C (0.03-0.20)

Click here to reset to form defaults

Outputs

Optimum Velocity (u*_e, cm/s) Ψ

Optimum Flow Rate (mL/min) λ

Optimum Column Length (L*, cm) η (Pa*s)

Optimized Plate Count (N*) ν

van Deemter Optimum Velocity (u_e,opt, cm/s) h

Particle Diameter Crossovers (t_o, s) H (cm)

1.8/3.5 ε_t

3.5/5.0 D_m at T

a) Column permeability based on the interstitial velocity; typical range is 500 to 1000

© Copyright 2008, Dwight R. Stoll, Peter W. Carr, and Xiaoli Wang, All Rights Reserved

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visits since October 1, 2008

Primary	#1 - (Red)	#2 - (Blue)	Secondary	#1	#2
Column Temperature (^oC)			Column Permeability^a (Φ)
Maximum Pressure (bar)			Interstitial Porosity (ε_e, 0-1)
Particle Size (μm)			Intraparticle Porosity (ε_i, 0-1)
Column Dead Time (t_o, s)
Column Diameter (mm)			van Deemter Coefficients (in reduced terms)
			A (1-2)
Organic Modfier Fraction (MeCN, 0-1)			B (3-20)
Analyte Diffusion Coef. @ 40 ^oC (cm²/s)			C (0.03-0.20)

Optimum Velocity (u*_e, cm/s)			Ψ
Optimum Flow Rate (mL/min)			λ
Optimum Column Length (L*, cm)			η (Pa*s)
Optimized Plate Count (N*)			ν
van Deemter Optimum Velocity (u_e,opt, cm/s)			h
Particle Diameter Crossovers (t_o, s)			H (cm)
1.8/3.5			ε_t
3.5/5.0			D_m at T