Exercise 1.11 - The Transmission Electron Microscope

LEVEL III

Figure 1.11 Zeiss EM9-S transmission electron microscope

Materials

• Transmission electron microscope facility

Procedure

1. Visit an electron microscope facility.

2. Familiarize yourself with the basic operations of Fixation, embedding and sectioning of materials for viewing in an electron microscope.

   If permitted, identify the primary parts of the TEM, including is various controls and operation. Refer to Figure 1.11 for details of the Zeiss EM9-S microscope.

3. Complete the following operations.
   • Insert and Remove a Specimen from the chamber.

   • Turn on the high voltage, check current levels and turn on the beam.

   • Find and center the beam.

   • Use magnification controls and condenser.

   • Use binocular viewing screen

   • Take an electron photomicrograph.

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