Chapter 10: Chromosomes

Table 10.1 Properties of chromatin


Morphotype
Activated chromatin Euchromatin
Non-activated chromatin Facultative and obligate heterochromatin, euchromatin
Structural organization
Less condensed, unfolding of functional domains (2040 kbp) exhibit
Highly condensed
DNA methylation (CG sites)
DNase I sensitive sites m^v-sites unmethylated
m^v-sites methylated
Nucleosomes
DNase I sensitive
DNase I resistant
Histones
H1-deprivated; core histones highly acetylated
H1-enriched; association with special H1 isofores, e.g., H5; H2A/H2B under-acetylated; eventually H2A modified by ubiquitin
HMG 14/17
Present
Absent
HMG 1/2
Present
Absent
Transcription RNAP/RNP
Presence of RNAP and RNP depends on the actual transcription state
No


Cell Biology Laboratory Manual
Dr. William H. Heidcamp, Biology Department, Gustavus Adolphus College,
St. Peter, MN 56082 -- cellab@gac.edu