Chapter 12: Cell Cultures - Endnotes
Harrison, R.G. (1907) Observations on the living
developing nerve fiber. Proc. Soc. Exp. Biol. Med. 4:140-143.
Carrel, A. (1912) On the permanent life of tissues
outside the organism. J. Exp. Med. 15:516-528.
One of the continuing differences between microbiologists and
microscopists is the lack of a coverslip when viewing bacteria.
Convenience causes microbiologists to skip the process of placing
mounting media and a coverslip on their slides. This causes
microscopists to cringe at the thought!
If the cultures are to remain aseptic, the cuvettes can be
sterilized and plugged. Alternatively, culture flasks with
cuvette side arms can be used.
Absorbance may be read directly if the spectrophotometer is
equipped with digital display. Absorbance is more difficult to
interpret on an analog display.
It is assumed that the media has been pre-mixed, with
serum and other additives put into the media. The media should be
in small aseptic containers for student use.
From: Barbara B. Mischell and Stanley M. Shiigi. "Selected Methods
in Cellular Immunity". W.H.Freeman & Co. San Francisco, 1980, p. 17.
Modified from Freshney, R. Ian. Culture of Animal Cells: A
Manual of Basic Technique. Alan R. Liss, Inc. New York, 1983.
Return to Table of Contents
Cell Biology Laboratory Manual
Dr. William H. Heidcamp, Biology Department, Gustavus Adolphus College,
St. Peter, MN 56082 -- firstname.lastname@example.org