| Minimal Essential Media | |||||
|---|---|---|---|---|---|
| Inorganic salts (mg/liter) |
|||||
CaCl |
200.00 |
KCl |
400.00 |
MgSO 7H O |
200.00 |
| NaCl |
6800.00 |
NaHCO |
2200.00 |
NaHPO H O |
140.00 |
| Other components |
|||||
| D-Glucose |
1000.00 |
Lipoic acid |
0.20 |
Phenol red |
10.00 |
| Sodium pyruvate |
110.00 |
|
|
|
|
| Amino Acids |
|||||
| L-Alanine |
25.00 |
L-Arginine |
105.00 |
L-Asparagine |
50.00 |
| L-Aspartic acid |
30.00 |
L-Cystine |
24.00 |
L-Cysteine |
100.00 |
| L-Glutamic acid |
75.00 |
L-Glutamine |
292.00 |
Glycine |
50.00 |
| L-Histidine |
31.00 |
L-Isoleucine |
52.50 |
L-Leucine |
52.40 |
| L-Lysine |
58.00 |
L-Methionine |
15.00 |
Phenylalanine |
32.00 |
| L-Proline |
40.00 |
L-Serine |
25.00 |
L-Threonine |
48.00 |
| L-Tryptophan |
10.00 |
L-Tyrosine |
36.00 |
L-Valine |
46.00 |
| Vitamins |
|||||
| Ca pantothenate |
1.00 |
Choline chloride |
1.00 |
Folic Acid |
1.00 |
| i-Inositol |
2.00 |
Nicotinamide |
1.00 |
Pyridoxal HCl |
1.00 |
| Riboflavin |
0.10 |
Thiamine HCl |
1.00 |
|
|
| Nutrient Broth | |||||
| |
g/liter |
||||
| Bacto-Beef Extract |
3 |
||||
| Bacto-Peptone |
5 |
||||
Source: GIBCO Laboratories Catalogue & Reference Guide, 1987 and DIFCO Manual of Dehydrated Culture Media and Reagents, DIFCO Laboratories, Detroit, MI.