Table 12.1 Eukaryote and prokaryote culture media


Minimal Essential Media
Inorganic salts (mg/liter)
CaCl_2
200.00
KCl
400.00
MgSO_47H_2O
200.00
NaCl
6800.00
NaHCO_3
2200.00
NaHPO_4H_2O
140.00
Other components
D-Glucose
1000.00
Lipoic acid
0.20
Phenol red
10.00
Sodium pyruvate
110.00




Amino Acids
L-Alanine
25.00
L-Arginine
105.00
L-Asparagine
50.00
L-Aspartic acid
30.00
L-Cystine
24.00
L-Cysteine
100.00
L-Glutamic acid
75.00
L-Glutamine
292.00
Glycine
50.00
L-Histidine
31.00
L-Isoleucine
52.50
L-Leucine
52.40
L-Lysine
58.00
L-Methionine
15.00
Phenylalanine
32.00
L-Proline
40.00
L-Serine
25.00
L-Threonine
48.00
L-Tryptophan
10.00
L-Tyrosine
36.00
L-Valine
46.00
Vitamins
Ca pantothenate
1.00
Choline chloride
1.00
Folic Acid
1.00
i-Inositol
2.00
Nicotinamide
1.00
Pyridoxal HCl
1.00
Riboflavin
0.10
Thiamine HCl
1.00


Nutrient Broth

g/liter
Bacto-Beef Extract
3
Bacto-Peptone
5

Source: GIBCO Laboratories Catalogue & Reference Guide, 1987 and DIFCO Manual of Dehydrated Culture Media and Reagents, DIFCO Laboratories, Detroit, MI.


Cell Biology Laboratory Manual
Dr. William H. Heidcamp, Biology Department, Gustavus Adolphus College,
St. Peter, MN 56082 -- cellab@gac.edu