Exercise 13.7 - Isolation of Fruiting Bodies without slugs



Procedure 27

  1. Cultures grown for 24-30 hours (agar or suspension) are washed free of bacteria by several centrifugations in fresh, cold water (2 minutes @ 300 Xg each).

  2. Apply the cells at a density of 6 X 10^6 cells per cm^2 to a water washed black Millipore filter supported by two cellulose absorbent pads previously saturated with LPS Buffer.

  3. Allow the cells to develop in the dark at 22° C in a humidity saturated chamber.

  4. Periodically withdraw a sample of the amoeba and sketch the development of the aggregates. Note in particular the induction of synchronous fruiting without the formation of migrating slugs.

Return to Table of Contents

Cell Biology Laboratory Manual
Dr. William H. Heidcamp, Biology Department, Gustavus Adolphus College,
St. Peter, MN 56082 -- cellab@gac.edu