Chapter 2: Histochemistry - Endnotes


  1. Sections may be cut directly from frozen blocks, if a cryostat is available. The sections should be fixed for 2 minutes in acid-alcohol, rehydrated and the procedure then begun with the amylase treatment (Step 4).

  2. Frozen sections fixed in acid-alcohol may be substituted. Begin processing with distilled water wash (Step 2).

  3. A Series 2 Wratten Filter with 15 watt bulb, which is at least 5 feet from the work area may be used, but reference should be made to the emulsion manufacturer's directions for safelight.

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Cell Biology Laboratory Manual
Dr. William H. Heidcamp, Biology Department, Gustavus Adolphus College,
St. Peter, MN 56082 -- cellab@gac.edu