Exercise 3.2 - Sedimentation Densities

LEVEL I

Materials

• Table 3.2 Density and refractive indexes of sucrose
  

• Table 3.1 Densities of cell structures in sucrose

• Sucrose density fractions from Exercise 3.1

Procedure

1. Using the densities for sucrose given in Table 3.2 7, compute the density of each organelle found within the fractions collected by differential centrifugation.

2. Compare these values with those listed in Table 3.1. 8

It is possible to approximate the densities of the particles separated above, based on the linear gradient separation data. It would be better to perform an additional centrifugation using CsCl as the viscous media, but for our purposes, the density of the sucrose will be sufficient.

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