Chapter 5: Enzymes - Endnotes
Numerical order or enzymes as classified by the
International Union of Biochemistry.
N.H. Horowitz, M. Gling and G. Horn. Tyrosinase (Neurospora crassa)
in Methods in Enzymology, Vol. XVII. (H. Tabor and C.W. Tabor eds.) p. 615-
S.H. Pomerantz and J.P. Li, Tyrosinase (Hamster Melanoma) in Methods
in Enzymology, Vol XII, p 620-626.
If the extraction of tyrosinase is to be
skipped, a commercial preparation of 0.7 micrograms of tyrosinase
(4,000 units/mg) in 4 ml of citrate buffer may be substituted.
This can be easily accomplished with many standard "scientific"
calculators, most computer spreadsheets, or with the BASIC program
listed in Appendix
The exercise is easier to perform if a temperature controlled
spectrophotometer is available, since the reaction rate can be
monitored at the proper temperature. Lacking this piece of equipment,
however, the reaction can be placed in an ice bath or incubator and
monitored every 30 seconds.
Conduit, University of Iowa, Oakdale Campus, Iowa City, Iowa 52242.
BIOSOFT, PO Box 580, Milltown, NJ 08850.
Note that the table lists the concentrations as those of the initial
substrate. This is not technically correct since the addition of 0.5
ml of enzyme will dilute the substrate. If Exercise 5.9 is to be
performed, the values of 1/s will need to be adjusted accordingly.
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Cell Biology Laboratory Manual
Dr. William H. Heidcamp, Biology Department, Gustavus Adolphus College,
St. Peter, MN 56082 -- email@example.com