You will note that for the determination of pH and temperature effects, we established conditions that scanned a wide range of the variables. To refine these procedures, we would add more values, for example, temperatures from 30- 40° C at steps of 1.0° C or 36-37° C with a step of 0.1° C. This is time consuming.
To establish the principle, however, let's shift to computer simulation of enzyme activity. Computer simulations will also assist in the rapid accumulation of data for kinetic analysis.
There are several excellent commercial programs available for enzyme kinetic analysis, and use of these must be left to the descretion of the instructor. The author has had excellent results with a rather old BASIC program, ENZKIN, which is still available from Conduit. 7
This is a simple, straight-forward and inexpensive program which is available for Apple Computers and for main frames. It can readily be converted to run on other machines with some simple source code changes. Unfortunately, Conduit may not continue supplying this program in the future.
If you do not wish to convert programs, or if you prefer a more sophisticated program is desired, the author recommends ENZPACK Ver. 2.0. 8 In addition to simulation of enzyme kinetics, this program allows entry of data with subsequent graphing and analysis. It also allows for high order kinetic analysis in addition to Lineweaver-Burk plots.
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