Chapter 7: Microsomes

7.5 Measurement of catecholamines

LEVEL III


Figure 7.6 Catecholamine metabolism

The function of chromaffin granules is the synthesis of the catecholamines. Thus, we can analyze chromaffin granule function by the level of catecholamines found in isolated granules; in particular dopamine, epinephrine and/or norepinephrine can be monitored. Unfortunately, these compounds are somewhat difficult to measure. While there are several means of monitoring these compounds, 12 the primary procedure requires the use of radio-immunoassay. 13

As a reference, figure 7.6 illustrates the interaction of the catecholamine hormones..

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Cell Biology Laboratory Manual
Dr. William H. Heidcamp, Biology Department, Gustavus Adolphus College,
St. Peter, MN 56082 -- cellab@gac.edu