Chapter 7 Microsomes
- FOOTNOTE 4:J.B. Schenkman and D.L. Dinti Preparation of
Microsomes with Calcium in METHODS IN ENZYMOLOGY, LII, PART C
(S. Fleisilen and L. Packer, eds.) Academic Press, New York,
1978. pp83-89. An alternative, and more lengthy procedure for the
preparation of lysosomes follows folows the techniques of Sawant
et al (Sawant, P.L., S. Shibko, U.S. Kumta and A.L. Tappel, 1964.
BIOCHIM BIOPHYS ACTA 85,82.). This alternative will yield a purer
lysosome fraction, but requires many more centrifugation steps.
The procedure listed in this exercise takes advantage of the fact
that lysosomes will aggregate in the presence of Ca&*SUPERSCRIPT
++. This procedure has the advantage of using only a preparative
centrifuge, while reducing the number of centrifugation steps.
Cell Biology Laboratory Manual