Chapter 7: Microsomes

Table 7.1 Physical properties of microsomes




Mitochondria
Lysosome
Perosisome
Identifying enzyme
Relative volume
(cm^3/g dry matrix)
Cytochrome oxidase
Acid phosphatase
Urate oxidase
Matrix
0.760
0.769
0.756
Hydration
0.430
0.256
0.214
Sucrose space
0.905
1.075
2.510
Osmotic space
0.595
0.485
0
Total
2.690
2.585
3.480
Relative mass (g/g dry matrix)



Matrix
1.000
1.000
1.000
Hydration
0.430
0.256
0.214
Sucrose space
0.936
1.112
2.596
Osmotic space
0.595
0.485
0
Total
2.961
2.853
3.810
Density
1.099
1.103
1.095
S 0.25 M/S 0.70 M sucrose 2.74
2.62
2.62

Cell Biology Laboratory Manual
Dr. William H. Heidcamp, Biology Department, Gustavus Adolphus College,
St. Peter, MN 56082 -- cellab@gac.edu