Chapter 7: Microsomes

Table 7.2 Chemical components of microsomes


Composition
RNA, ribosomal and others
Protein (variable), both enzymes and structural
Lipids (30-50% of composition weight)
Phospholipids
Phosphatidylserine
Cholesterol
Lecithins
Phosphatidyl inositol
Unsaturated Fatty acids
Carbohydrate metabolism
Glucose-6-phosphatase
UDP-glucose dephosporylation
L-ascorbic acid synthesis
UDP-glycuronic acid metabolism
Protein metabolism
Several enzymes for protein synthesis
Iodine incorporation enzymes
Peptidases, amidases
Fatty acid metabolism
Reductive synthesis of short chain fatty acids
Acylation with Acyl-CoA (ligase)
Synthesis of glycolipids and plasmalogens
Neutral glyceride synthesis
Phospholipid metabolism
Phosphatidic acid synthesis
Decarboxylation of phosphatidylserine
Redistribution of phosphatides in membranes
Phosphatidylcholine synthesis
Transmethylation of phosphatides
Steroid metabolism
Synthesis of cholesterol from acetate
Interconversion and degradation of steroids
Hydroxylations, aromatizations, dehydrogenases and hydroxylases
Nucleotide metabolism
Nucleoside diphosphatases
NAD and NADH pyrophosphatase, NADase
5'-Nucleotidases
Oxidation-Reduction
NADH and NADPH cytochrome C reductase
Nonspecific and NADP diaphorase
Pyridine nucleotides
Cytochromes, P450, coenzyme Q
Transport and Transmission
Sarcoplasmic AMPases
Cholinesterase
Mg++ Ca++ dependent AMPases
Drug detoxication
N- and O- deacylations
N-demethylation, sidechain and thio-ester oxidation
Desulphuration, deamination and deiodination
Hydroxylation of azo dyes
Binding properties
Azo dyes
Iodide
Epinephrine, norepinephrine

Cell Biology Laboratory Manual
Dr. William H. Heidcamp, Biology Department, Gustavus Adolphus College,
St. Peter, MN 56082 -- cellab@gac.edu