Exercise 9.1 - Fluorescent Analysis of Microtubules
Figure 9.1 Macrophage stained with FITC labeled anti-tubulin
- Projection slides of tubulin fluorescence1
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- Draw the cells represented in Figure 9.1.
These are photographs of macrophages treated with a fluorescent
- Describe the shape and general appearance of the cells
observed in the first three slides. How does the organization of
the microtubules affect cell shape?
- Identify a place in the cell from which the microtubules
appear to extend. This is the microtubule-organizing center.
Label it in the appropriate drawings.
- The cells in slide 4 were exposed to colchicine for 30 minutes
and then prepared as above. What do you predict will happen to
the microtubules after cell treatment with colchicine? Was this
prediction fulfilled? Draw a colchicine-treated cell.
- The cells in slide 5 were placed on ice for 30 minutes and then
prepared as above. What do you predict will happen to the
microtubules after incubation of the cells in the cold? Is the
microtubule diameter larger or smaller than the resolving power
of the light microscope? How can microtubules be visualized with
immunofluorescence if the tubules are less than the resolution of
the light microscope?
Cell Biology Laboratory Manual
Dr. William H. Heidcamp, Biology Department, Gustavus Adolphus College,
St. Peter, MN 56082 -- email@example.com