Synthetic yeast media lacking uracil

1. Add the following to 3/4th's the final volume of water:

2.0 grams/liter uracil minus drop-out base (USBiological D9536)
6.7 grams/liter yeast nitrogen base (USBiological Y2025)
20.0 grams/liter carbon source (2% w/v)*, either D(+)glucose (USBiological G3050) or D(+)galactose (USBiological G1030)
2. Bring the solutioon to its final volume.

3. Sterilize media before use.

* To test for growth on a non-fermentable carbon source, substitute 3% (v/v) glycerol for glucose/galactose, i.e. 30 ml glycerol/liter.