CHE380 - Instrumental Methods of Analysis
Fall 2019
Dr. Dwight Stoll

Stuff - Syllabus, Topic Outline and Objectives, Class Leadership Opportunities and Rubric, Study Questions Through Exam #1 (clean copy), Study Questions Through Exam #2 (clean copy), Study Questions Through the Final Exam (clean copy), Useful Links, Writing Guide

Textbook: Mixed sources

Weeks - 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15

Week 1 (top) (slides)
Date Day Course
Day
 Topic Reading Video Multimedia Handouts Notes
9/2
 M






9/3
 T
 1
 Learning to be Astonished
 Self Knowledge, from Consolations

Learning to be Astonished Reflection
9/4
 W
 2
 Introduction to 360/380 Mashup and Lab


Laboratory Project Prospectus
9/6
 F
 3
 Introduction to the Course


US Customs Crude Oil method

Week 2 (top)
Date Day Course
Day
 Topic Reading Homework
 Video Multimedia Handouts Notes
9/9
 M
 4
 Sample Preparation-1 Poole Chapter on SPE; Waters SPE Guide - Overview (pp. 18-26), Steps (101-111), Retention (41-51);
Introduction to Sample Preparation and SPE
  ChromAcademy - SPE: Molecular Properties, SPE Overview Sample Preparation Slides; SPE Examples
9/10
 T
 5
 Astonishment Discussion
 Pages from The Slow Professor




9/11
 W
 6
 Sample Preparation-2: LLE; QuECheRS; SPME
 		Practical Aspects of LLE; SPME - Overview, Original paper; QuECheRS - Inventor interview, AOAC method for spinach
Sample Preparation Part 3 - LLE; Part 4 - QuECheRS; Part 5 - SPME
 ChromAcademy  Liquid/Liquid Extraction Techniques; ChromAcademy - GC->Sampling Techniques -> SPME; Supelco website on SPME

9/13
 F
 7
 Sample Preparation-3: Contemporary Applications 







Week 3 (top)
Date Day Course
Day
 Topic Reading Homework
 Video Multimedia Handouts
9/16
 M
 8
 Quiz #1; Separations-1.1-Intro to Separations
 Vitha Book - Sections 1.1, 1.6-1.7
Separations Primer
All Separations Slides
9/17
 T
 19
 Narrative & Vocation
 (see 360 Moodle page) What is public narrative? by Marshall Ganz



9/18
 W
 10
 Separations-1.2-Retention in GC
 Hinshaw and Ettre Parts 1, 3, and 5
Separations-Part II-GC Retention
GC Hardware
9/20
 F
 11
 Separations-1.3-Retention in LC
 Modern HPLC for Practicing Scientists, Dong, Sections 1.2, 2.2, 2.3, and 2.6 Offline HW #1
 LC Retention - Empirical and Practical Knowledge; Thermodynamics of Retention in LC



Week 4 (top) (notes and slides)
Date Day Course
Day
 Topic Reading Video Multimedia Handouts
9/23
 M
 12
 Separations-1.4-LC Retention, cont.



9/24 T
Nobel Conference - No Class



9/25 W
Nobel Conference - No Class



9/27
 F
 13
 Antibodies and Their Techniques





Week 5 (top)
Date Day Course
Day
 Topic Reading Video Homework
 Multimedia Handouts
9/30
 M
 18
 Separations-1.5




10/1
 T
 19
 Deep Listening Recap and Resiliency Explored




10/2 W 20 Quiz #2; Separations-1.6-Wrap up Retention




10/4
 F
 21
 Separations-1.7-Contemporary Applications
Separations Primer





Week 6 (top)
Date Day Course
Day
 Topic Reading Video Homework
 Multimedia Handouts
10/7
 M
 18
 Mass Spec-1- Ionization (EI and ESI)
 MS Primer, Sections 1, 2 Primer-3-Mass Spectrometry

All MS Slides; MS Theory Slides; MS Hardware Slides
10/8
 T
 19
 Model for Resiliency




10/9 W 20 Mass Spec-2-Ionization, cont., and Analyzers (Q, TOF, FTICR) MS Primer, Balogh, Section 8; ChromAcademy -> MS -> Mass Analyzers



10/11
 F
 21
 Density, in vitro, and Fluor-Based Assays






Week 7 (top) (notes)
Date Day Course
Day
 Topic Reading Video Multimedia Handouts
10/14
 M
 22
 Exam #1



10/15 T
 23
 Self Care Assess, Resiliency Plan, Ego/Soul Stories



10/16
 W
 24
 Mass Spec-3-Analyzers cont., Tandem MS ChromAcademy -> MS -> Mass Analyzers; ChromAcademy Tutorial on MS/MS Analysis


10/18
 F
 25
 Mass Spec-4-Applications
Primer-3-Mass Spectrometry


Week 8 (top)

Date Day Course
Day
 Topic Reading Video Multimedia Handouts
10/21
 M
Fall Break



10/22 T




10/23*
 W
 26
 Visit from Career



10/25
 F
 27
 Separations-2.1-Physics of Column-Based Separations
 Random Walks in Biology, Chapter 1; Giddings' Random Walk paper; Hinshaw and Ettre, Part IV; Dong, Sections 2.2, 2.5


*DS at Merck in NJ.

Week 9 (top)
Date Day Course
Day
 Topic Reading Video Multimedia Handouts
10/28
 M
 28 Separations-2.2-Broadening in GC Hinshaw and Ettre, Part IV Broadening in GC Two-Stream Laminar Flow in Micro Device; Simulated Laminar Flow
10/29
 T
 29 Ego/Soul Stories / Seth Godin



10/30
 W
 30 Separations-2.3-Broadening in LC Dong, Sections 2.2, 2.5


011/1
 F
 31 Separations-2.4-Detectors for GC Hinshaw and Ettre, Part VIII




Week 10 (top)(slides, notes)
Date Day Course
Day
 Topic Reading Video Multimedia Handouts
11/4
 M
 32 Separations-2.5-Detectors for LC Dong, Sections 4.4-4.8


11/5
 T
 33 Ground - Soil & Place



11/6
 W
 34 Quiz #3; Spectroscopy-1
 Harvey AC2.1, Sections 10A-10C; Article on Basics of Quantitation


11/8
 F
 35 Spectroscopy-2 Harvey AC2.1, Sections 10C and 10F; Agilent Spectroscopy Primer




Week 11 (top)(slides, notes)
Date Day Course
Day
 Topic Reading Homework
 Video Multimedia Handouts
11/11
 M
 36
 Spectroscopy-3 Harvey AC2.1, Section 10C; Chapter from Skoog, Holler, and Crouch



11/12
 T
 37
 Ground - Soil & Place




11/13
 W
 38
 Spectroscopy-4




11/15
 F
 39
 Spectroscopy-5-Applications






Week 12 (top) (notes)
Date Day Course
Day
 Topic Reading Video Multimedia Handouts
11/18
 M
 40 Finish Spectroscopy; E&SP-1 - Basic electronics Rubinson Chapter 6, sections 6.1-6.4 and 6.8

All E&SP Slides
11/19
 T
 41 Resiliency #2



11/20
 W
 42 Exam #2



11/22
 F
 43 Poster Workshop





Week 13 (top)
Date Day Course
Day
 Topic Reading Video Multimedia Handouts
11/25
 M
 44
 E&SP-2- Loading Errors and Op-Amps Rubinson Section 6.8, 17.1 Loading Errors and Op-Amps

11/26
 T
 45
 Overview of Personal Narrative and Final



11/27
 W
 Thanksgiving




11/29
 F
 Thanksgiving






Week 14 (top)
Date Day Course
Day
 Topic Reading Video Multimedia Handouts
12/2 M
 46
 Quiz #4; E&SP-3 - Analog Filtering, A/D Conversion Rubinson and Rubinson, sections 6.5, 17.6, and 17.1 (Chapter 17) Analog Filtering

12/3 T
 47
 Echem-1 - Basics Harvey AC2.1, Sections 11A Overview of Echem for Quantitation; Nernst Equation
All Echem Slides
12/4 W
 48
 Echem-2 - Amperometry, Conductometry
 Harvey AC2.1, Sections 11D


12/6 F
 49
 Echem-3 - Coulometry, Potentiometry, Applications
 Harvey AC2.1, Sections 11B,C




Week 15 (top)
Date Day Course
Day
 Topic Reading Video Multimedia Handouts
12/9 M
 50
 E&SP-4-Applications Rubinson and Rubinson, sections 17.2, 17.4, 17.5, 17.7 (Chapter 17)


12/10 T
 51
 Capillary Electrophoresis
 Harvey AC 2.0, section 12G
Agilent Capillary Electrophoresis Primer


12/11
 W
 52
 Other Separations (IEX, HILIC, Chiral, SEC)
 Section 1.2 from Modern HPLC for Practicing Scientists, by Michael Dong       
Supelco Guide to HILIC Separations
CHROMacademy HILIC Tutorial
CHROMacademy HILIC Module
CHROMacademy NPLC Module
CHROMacademy IEX Tutorial       
CHROMacademy IEX Module
Section 1.2 from Modern HPLC for Practicing Scientists, by Michael Dong


12/13
 F
 53
 Final Exam




Week 16 (top)
Date Day Course
Day
 Topic Reading Video Multimedia Handouts
12/16 M
 54
 Final Joint Class Meeting